According to the current division there are six wine-growing regions in the Czech Republic. Two of these are located in Bohemia  – the Mělník and Litoměřice regions and four in Moravia: Znojmo in the western-most part, the Mikulov region in the south near the Austrian border, the Velké Pavlovice region in the central and northern part of the entire territory and Slovácko to the east. In the past this area was divided into ten small wine-growing sub-regions, namely Brno, Bzenec, Kyjov, Mikulov, Mutěnice, Podluží, Strážnice, Uherské Hradiště, Velké Pavlovice and Znojmo. All of these small sub-regions are situated next to each other creating one unified area in South Moravia.

Thanks to its western position the Znojmo Wine-growing Sub-region is partially under the influence of the climatic conditions of the Českomoravská vrchovina highlands, and its soils are more or less of a rocky nature, which are especially suitable for growing varieties that need somewhat harsh conditions for good results. That for example is Rhine Riesling, which is produced a lot in this territory. The centre of this area is undoubtedly the town of Znojmo, situated near the Austrian border, boasting a rich wine-growing tradition, documented for example by vast wine cellars or even corridors located under the actual town. In its vicinity there are excellent vineyards, a fact supported by famous names such as Kraví hora, Šatov, Vrbovec and others. Vineyards around Miroslav and Dolní Kounice, where blue varieties prosper, are also worth mentioning. An interesting representative of this area is for example a small wine-growing enterprise - Trpělka & Oulehla, producing wines, which have recently been gaining an ever growing popularity among wine consumers, and as is usual in Moravia, it mainly concerns white wines.

The town of Mikulov, also located not far from the Austrian border, is the centre of an important wine-growing region of the same name. Approximately, half way to Břeclav there is a town of Valtice, which is interesting thanks to having the oldest viticulture school in our country and the permanent wine tasting exposition of the Wine Salon of the Czech Republic which is the most important wine competition  in this country. The village of Pavlov is situated in the hills above the Nové Mlýny dam, boasting excellent conditions for white varieties, such as both types of Rieslings, Pinot Noir and Chardonnay. The wine cultivation station in Perná gave life to the Pálava variety. The most significant representatives of the Mikulov wine-growing region are for example the Mikrosvín winery, known for its strong white wines with a higher alcohol content, Reisten winery from Pavlov, concentrating almost exclusively on white wines or Tanzberg with vineyards above Mikulov.

South of Brno in the direction of the Austrian border stretches a belt of the main vineyards of the Velké Pavlovice Sub-region. It is a place where within the territory of Moravia red wines probably thrive best. That is why we can find here large areas growing blue varieties, such as Blauer Portugieser, Zweigeltrebe or St. Laurent, for example around Rakvice and Bořetice. Another attractive place of this area is Čejkovice, also thanks to its history, connected with viniculture. Vast cellars in Čejkovice built by the Knights Templar are definitely worth visiting. Interesting wine-growers of this area are represented by “small wine-maker” František Mádl from Velké Bílovice, who concentrates mainly on white wines and whose Barrique Sauvignon is traditionally successful, and Ing. Miloš Michlovský, who has some of his vineyards in this area.

The most eastern Slovácko Sub-region includes wine-growing towns such as Břeclav, Mutěnice, Kyjov, Bzenec, Strážnice, Uherské Hradiště and Blatnice, and obviously villages as well as vineyards adjoining them. Cultivation stations located in this area gave life to two local varieties, the most prevalent type in this country - Moravian Muscat and Cabernet Moravia, gained from crossing the Cabernet Frank and Zweigeltrebe varieties. Interesting wines in Slovácko are produced for example by wine-grower Michal Robek from Blatnice, naturally sweet wines come from Neoklas in Šardice while Spielberg offers interesting red wines.